Thermal and pH Stabilities of Partially Purified Polyphenol Oxidase Extracted from Solanum melongenas and Musa sapientum Fruits
نویسندگان
چکیده
منابع مشابه
Comparison of polyphenol oxidase in fruits of Solanum melongena L. (purple) and Solanum melongena L. (green)
A comparative study of polyphenol oxidase (PPO) from two varieties of brinjal (local long green-bili badnea and purple-gundu badne) was conducted to determine differences and similarities of some of the characteristics of the PPO enzymes in terms of temperature and pH optima, substrate specificity, thermal inactivation and potency of some PPO inhibitors. PPO was partially purified by ammonium s...
متن کاملEffect of Sodium Dodecyl Sulphate on Partial Purified Polyphenol Oxidase Activity in Red and Green Tomatoes (Solanum Lycopersicum)
In order to better understand how to prevent enzymatic greenning, it is important to understand kinetic properties of polyphenol oxidase. we investigated the effect of SDS on the rate of catechol oxidation by small cherry tomato partial purified PPO. PPO activity increased with increasing SDS concentration. The most effective concentration of SDS was 0.8, 1 and 1.25 mM in according to pH and ...
متن کاملPartial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...
متن کاملEffect of Sodium Dodecyl Sulphate on Partial Purified Polyphenol Oxidase Activity in Red and Green Tomatoes (Solanum Lycopersicum)
In order to better understand how to prevent enzymatic greenning, it is important to understand kinetic properties of polyphenol oxidase. we investigated the effect of SDS on the rate of catechol oxidation by small cherry tomato partial purified PPO. PPO activity increased with increasing SDS concentration. The most effective concentration of SDS was 0.8, 1 and 1.25 mM in according to pH and ...
متن کاملPartial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...
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ژورنال
عنوان ژورنال: American Journal of Plant Physiology
سال: 2013
ISSN: 1557-4539
DOI: 10.3923/ajpp.2013.63.73